Edward L. D'Antonio, Ph. D.Edward L. D'Antonio, Ph. D.

Associate Professor of Biochemistry and Structural Biology
University of South Carolina Beaufort
Science and Technology Building, Room 108
One University Boulevard, Bluffton, SC 29909
Office: 843-208-8101   Lab: 843-208-8167
Email: edantonio@uscb.edu
Website: www.facebook.com/DAntonio-Laboratory

Overview

Structural Biology, Biochemistry, Chagas' Disease, and Medicinal Chemistry.

Research

My team consists of undergraduate researchers that utilize a variety of techniques, including: analytical chemistry, biotechnology, enzymology, structural biology, and synthetic chemistry. I use the structure-based drug design methodology to develop new classes of competitive inhibitors for enzyme drug-targets found in parasites of trypanosomatid diseases.  These diseases include Chagas' disease (Trypanosoma cruzi), human African trypanosomiasis (Trypanosoma brucei), and leishmaniasis (Leishmania spp.).   I focus on glycolytic and pentose phosphate pathway enzymes, which are known to be critically important for the survival of these parasites.  Since human cells usually have similar enzymes of these drug-targets, our drug designs are created that give rise to parasite selectivity.  Another goal of my research is to optimize existing inhibitor designs by increasing molecular interactions within the inhibitor's binding site.

Education

  • B.S. in Chemistry, Millersville University (2003)
    Advisor: Dr. Lyman H. Rickard
  • Ph.D. in Chemistry, North Carolina State University (2010)
    Advisor: Dr. Edmond F. Bowden
  • Postdoctoral Fellowship in Structural Biology, University of Pennsylvania (2010-2013)
    Advisor: Dr. David W. Christianson

Courses

  • Principles of Biochemistry I
  • Quantitative Analysis
  • Forensic Techniques and Data Analysis
  • General Chemistry I & II
  • Biological Principles I
  • Seminar in Biochemistry Research (Survey of Drug Discovery Methodologies)

About the Research Group on Neglected Tropical Diseases

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Publications and Patents

[19]  Mercaldi, G. F., D'Antonio, E. L., Aguessi, A., Rodriguez, A., and Cordeiro, A. T.
         (2019) Discovery of antichagasic inhibitors by high-throughput screening with
         Trypanosoma cruzi glucokinase. Bioorg. Med. Chem. Letters, 29, 1948-1953.
         DOI: 10.1016/j.bmcl.2019.05.037

[18]  D'Antonio, E. L. (2019) Monosaccharide amine and 3-nitro-2-phenyl-2H-chromene
         based inhibitors of glucose kinases.  U.S. Patent Application No. US 62/840,005.

[17]  Buechner, G. S., Millington, M. E., Perry, K., and D'Antonio, E. L. (2019) The crystal
         structure of glucokinase from Leishmania braziliensis. Mol. Biochem. Parasitol.
         227, 47-52.  DOI: 10.1016/j.molbiopara.2018.12.002

[16]  D'Antonio, E. L. (2018) Inhibitors of glucose kinases, along with methods of
         their formation and use.  U.S. Patent Application No. US 2018/0280404 A1.

[15]  D'Antonio, E. L. (2019) Cysteine-modifying substrate analogue inhibitors of ribose
         5-phosphate isomerase for parasitic diseases, along with methods of their formation
         and use.  U.S. Patent No. US 10,336,777 B2
.

[14]  D'Antonio, E. L. and D'Antonio, J. (2018) Therapeutic monosaccharide-based
         inhibitors of hexokinase and glucokinase for parasitic diseases, along with methods
         of their formation and use.  U.S. Patent No. US 9,956,240 B2
.

[13]  D'Antonio, E. L., Deinema, M. S., Kearns, S. P., Frey, T. A., Tanghe, S., Perry, K.
         Roy, T. A., Gracz, H. S., Rodriguez, A., and D'Antonio, J. (2015) Structure-based
         approach to the identification of a novel group of selective glucosamine analogue
         inhibitors of Trypanosoma cruzi glucokinase. Mol. Biochem. Parasitol. 204, 64-76.
         DOI: 10.1016/j.molbiopara.2015.12.004.

[12]  Taratula, O., Bai, Y., D'Antonio, E. L., and Dmochowski, I. J. (2014) Enantiopure
         cryptophane-129Xe nuclear magnetic resonance biosensors targeting
         carbonic anhydrase. Supramol. Chem. 1-8, DOI: 10.1080/10610278.2014.906601.
   
[11]  Chen, M., Al-lami, N., Janvier, M., D'Antonio, E. L., Faraldos, J. A., Cane,
         D. E., Allemann, R. K., and Christianson, D. W. (2013) Mechanistic insights
         from the binding of substrate and carbocation intermediate analogues to aristolochene
         synthase. Biochemistry, 52, 5441-5453.
         PMCID: PMC3755762.

[10]  D'Antonio, E. L., Ullman, B., Roberts, S. C., Gaur Dixit, U., Wilson, M. E.,
         Hai, Y., and Christianson, D. W. (2013) Crystal structure of arginase from
         Leishmania mexicana and implications for the inhibition of polyamine biosynthesis
         in parasitic infections. Arch. Biochem. Biophys., 535, 163-176.
         PMCID: PMC3683356.
    
[9]    Genshaft, A., Moser, J-A., D'Antonio, E. L., Bowman, C., and Christianson, D. W.
         (2013) Energetically unfavorable amide conformations for N6-acetyllysine side chains
         in refined protein structures. Proteins: Struct., Funct., Bioinf., 81, 1051-1057.
         PMCID: PMC3659166.
  
[8]    D'Antonio, E. L., Chen, T. K., Turner, A. H., Santiago-Capeles, L., and
         Bowden, E. F. (2013) Voltammetry of dehaloperoxidase on self-assembled monolayers:
         reversible adsorptive immobilization of a globin. Electrochem. Commun., 26, 67-70.
  
[7]    D'Antonio, E. L., Hai, Y., and Christianson, D. W. (2012) Structure and function of
         non-native metal clusters in human arginase I. Biochemistry, 51, 8399-8409.
         PMCID: PMC3490219.
   
[6]    D'Antonio, E. L. and Christianson, D. W. (2012) Binding of the unreactive substrate
         analog L-2-amino-3-guanidinopropionic acid (dinor-L-arginine) to human arginase I.
         Acta Crystallogr., F68, 889-893.
         PMCID: PMC3412766.
   
[5]    D'Antonio, E. L., Bowden, E. F., and Franzen, S. (2012) Thin-layer
         spectroelectrochemistry of the Fe(III)/Fe(II) redox reaction of dehaloperoxidase-hemoglobin.
         J. Electroanal. Chem., 668, 37-43.
   
[4]    D'Antonio, E. L., D'Antonio, J., de Serrano, V., Gracz, H., Thompson, M. K.,
         Ghiladi, R. A., Bowden, E. F., and Franzen, S. (2011) Functional consequences
         of the creation of an Asp-His-Fe triad in a 3/3 globin. Biochemistry, 50, 9664-9680.
         PMCID: PMC4007314.
   
[3]    D'Antonio, E. L. and Christianson, D. W. (2011) Crystal structures of complexes
         with cobalt-reconstituted human arginase I. Biochemistry, 50, 8018-8027.
         PMCID: PMC3172387.
    
[2]    D'Antonio, J., D'Antonio, E. L., Thompson, M. K., Bowden, E. F., Franzen, S.,
         Smirnova, T., Ghiladi, R. A. (2010) Spectroscopic and mechanistic investigations
         of dehaloperoxidase B from Amphitrite ornata. Biochemistry, 49, 6600-6616.
         PMCID: PMC2921985.

[1]    de Serrano, V., Davis, M. F., Gaff, J. F., Zhang, Q., D'Antonio, E. L., Bowden, E. F.,
         Rose, R., and Franzen, S. (2010) X-ray structure of the metcyano form of
         dehaloperoxidase from Amphitrite ornata. Acta Crystallogr., D66, 770-782.
         PMID: 20606257.

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