Edward L. D'Antonio, Ph. D.Edward L. D'Antonio, Ph. D.

Associate Professor of Biochemistry and Structural Biology
University of South Carolina Beaufort
Science and Technology Building, Room 108
One University Boulevard, Bluffton, SC 29909
Office: 843-208-8101   Lab: 843-208-8167
Email: edantonio@uscb.edu
Website: www.facebook.com/DAntonio-Laboratory


Biochemistry, Structural Biology, Medicinal Chemistry, High-Throughput Screening, SARS-CoV-2, Betacoronaviruses, COVID-19, Chagas' Disease, Leishmaniasis, Human African Trypanosomiasis, and Neglected Tropical Diseases.


Undergraduate researchers in my laboratory utilize a variety of important techniques ranging from analytical chemistry, biotechnology, enzymology, structural biology, compound screening, and synthetic chemistry. We implement structure-based drug design and screening approaches in order to develop or identify new classes of inhibitors for protein targets found in parasites that cause trypanosomatid diseases and the Coronavirus Disease 2019 (COVID-19).


  • B.S. in Chemistry, Millersville University (2003)
    Advisor: Dr. Lyman H. Rickard
  • Ph.D. in Chemistry, North Carolina State University (2010)
    Advisor: Dr. Edmond F. Bowden
  • Postdoctoral Fellowship in Structural Biology, University of Pennsylvania (2010-2013)
    Advisor: Dr. David W. Christianson


  • Principles of Biochemistry I
  • Quantitative Analysis
  • Forensic Techniques and Data Analysis
  • General Chemistry I & II
  • Biological Principles I
  • Seminar in Biochemistry Research (Survey of Drug Discovery Methodologies)

Research on Drug Discovery to Fight Off Disease

Personnel & Alumni
Group Photos
CV of PI
Media (Presentations & Interviews)


Publications and Patents

[22]  D'Antonio, E. L. (2021) Monosaccharide amine and 3-nitro-2-phenyl-2H-chromene
         based inhibitors of glucose kinases.  U.S. Patent No. US 11,059,842 B2.

[21]  Green, S. B., Lanier, R. J., Jr., Carey, S. M., Morgan, D. R., Gracz, H., Sherman, J.,
         Rodriguez, A., and D'Antonio, E. L. (2021) Synthesis, biochemical, and biological
         evaluation of C2 linkage derivatives of amino sugars, inhibitors of glucokinase
         from Trypanosoma cruzi. Bioorg. Med. Chem. Lett. 47, 128227.
         DOI: 10.1016/j.bmcl.2021.128227

[20]  Gonzalez, S. N., Mills, J. J., Maugeri, D., Olaya, C., Laguera, B. L., Enders, J. R.,
         Sherman, J., Rodriguez, A., Pierce, J. G., Cazzulo, J. J., and D’Antonio, E. L.
         (2021) Design, synthesis, and evaluation of substrate – analogue inhibitors of
         Trypanosoma cruzi ribose 5-phosphate isomerase type B, Bioorg. Med. Chem. Lett.
         32, 127723.  DOI: 10.1016/j.bmcl.2020.127723

[19]  D'Antonio, E. L. and Pierce, J. G. (2020) One-step synthesis of phosphate-based inhibitors
         and applications thereof.  U.S. Patent Application No. US 17/005,917.

[18]  D'Antonio, E. L. (2020) Inhibitors of glucose kinases, along with methods of
         their formation and use.  U.S. Patent  No. US 10,682,359 B2.

[17]  Mercaldi, G. F., D'Antonio, E. L., Aguessi, A., Rodriguez, A., and Cordeiro, A. T.
         (2019) Discovery of antichagasic inhibitors by high-throughput screening with
         Trypanosoma cruzi glucokinase. Bioorg. Med. Chem. Lett. 29, 1948-1953.
         DOI: 10.1016/j.bmcl.2019.05.037

[16]  Buechner, G. S., Millington, M. E., Perry, K., and D'Antonio, E. L. (2019) The crystal
         structure of glucokinase from Leishmania braziliensis. Mol. Biochem. Parasitol.
         227, 47-52.  DOI: 10.1016/j.molbiopara.2018.12.002

[15]  D'Antonio, E. L. (2019) Cysteine-modifying substrate analogue inhibitors of ribose
         5-phosphate isomerase for parasitic diseases, along with methods of their formation
         and use.  U.S. Patent No. US 10,336,777 B2.

[14]  D'Antonio, E. L. and D'Antonio, J. (2018) Therapeutic monosaccharide-based
         inhibitors of hexokinase and glucokinase for parasitic diseases, along with methods
         of their formation and use.  U.S. Patent No. US 9,956,240 B2

[13]  D'Antonio, E. L., Deinema, M. S., Kearns, S. P., Frey, T. A., Tanghe, S., Perry, K.
         Roy, T. A., Gracz, H. S., Rodriguez, A., and D'Antonio, J. (2015) Structure-based
         approach to the identification of a novel group of selective glucosamine analogue
         inhibitors of Trypanosoma cruzi glucokinase. Mol. Biochem. Parasitol. 204, 64-76.
         DOI: 10.1016/j.molbiopara.2015.12.004.

[12]  Taratula, O., Bai, Y., D'Antonio, E. L., and Dmochowski, I. J. (2014) Enantiopure
         cryptophane-129Xe nuclear magnetic resonance biosensors targeting
         carbonic anhydrase. Supramol. Chem. 1-8, DOI: 10.1080/10610278.2014.906601.
[11]  Chen, M., Al-lami, N., Janvier, M., D'Antonio, E. L., Faraldos, J. A., Cane,
         D. E., Allemann, R. K., and Christianson, D. W. (2013) Mechanistic insights
         from the binding of substrate and carbocation intermediate analogues to aristolochene
         synthase. Biochemistry, 52, 5441-5453.
         PMCID: PMC3755762.

[10]  D'Antonio, E. L., Ullman, B., Roberts, S. C., Gaur Dixit, U., Wilson, M. E.,
         Hai, Y., and Christianson, D. W. (2013) Crystal structure of arginase from
         Leishmania mexicana and implications for the inhibition of polyamine biosynthesis
         in parasitic infections. Arch. Biochem. Biophys., 535, 163-176.
         PMCID: PMC3683356.
[9]    Genshaft, A., Moser, J-A., D'Antonio, E. L., Bowman, C., and Christianson, D. W.
         (2013) Energetically unfavorable amide conformations for N6-acetyllysine side chains
         in refined protein structures. Proteins: Struct., Funct., Bioinf., 81, 1051-1057.
         PMCID: PMC3659166.
[8]    D'Antonio, E. L., Chen, T. K., Turner, A. H., Santiago-Capeles, L., and
         Bowden, E. F. (2013) Voltammetry of dehaloperoxidase on self-assembled monolayers:
         reversible adsorptive immobilization of a globin. Electrochem. Commun., 26, 67-70.
[7]    D'Antonio, E. L., Hai, Y., and Christianson, D. W. (2012) Structure and function of
         non-native metal clusters in human arginase I. Biochemistry, 51, 8399-8409.
         PMCID: PMC3490219.
[6]    D'Antonio, E. L. and Christianson, D. W. (2012) Binding of the unreactive substrate
         analog L-2-amino-3-guanidinopropionic acid (dinor-L-arginine) to human arginase I.
         Acta Crystallogr., F68, 889-893.
         PMCID: PMC3412766.
[5]    D'Antonio, E. L., Bowden, E. F., and Franzen, S. (2012) Thin-layer
         spectroelectrochemistry of the Fe(III)/Fe(II) redox reaction of dehaloperoxidase-hemoglobin.
         J. Electroanal. Chem., 668, 37-43.
[4]    D'Antonio, E. L., D'Antonio, J., de Serrano, V., Gracz, H., Thompson, M. K.,
         Ghiladi, R. A., Bowden, E. F., and Franzen, S. (2011) Functional consequences
         of the creation of an Asp-His-Fe triad in a 3/3 globin. Biochemistry, 50, 9664-9680.
         PMCID: PMC4007314.
[3]    D'Antonio, E. L. and Christianson, D. W. (2011) Crystal structures of complexes
         with cobalt-reconstituted human arginase I. Biochemistry, 50, 8018-8027.
         PMCID: PMC3172387.
[2]    D'Antonio, J., D'Antonio, E. L., Thompson, M. K., Bowden, E. F., Franzen, S.,
         Smirnova, T., Ghiladi, R. A. (2010) Spectroscopic and mechanistic investigations
         of dehaloperoxidase B from Amphitrite ornata. Biochemistry, 49, 6600-6616.
         PMCID: PMC2921985.

[1]    de Serrano, V., Davis, M. F., Gaff, J. F., Zhang, Q., D'Antonio, E. L., Bowden, E. F.,
         Rose, R., and Franzen, S. (2010) X-ray structure of the metcyano form of
         dehaloperoxidase from Amphitrite ornata. Acta Crystallogr., D66, 770-782.
         PMID: 20606257.

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